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To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles
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Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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. and hence this wavelength was chosen as the emission wavelength for the isothermal denaturation melts
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. The isolated EIN is a monomer, which cannot be phosphorylated by PEP, but participates in a reversible phosphorylation reaction with phosphorylated HPr (2,6)
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. Additional dehydration of monocations can occur inside DNA grooves as a result of deformation of DNA secondary structure
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. 6 as a horizontal line indicated with the letter "U" in different colors corresponding to the probe
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. However, viscosity alone does not give the complete picture, since we also note a significant blue shift in the fluorescence maximum when bound to amyloid fibrils and to prefibrillar misfolded oligomers
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. However, we obtained no useful information on the glass transition temperature T^sub g^
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