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The dashed curve in Fig
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For all heating rates the secondary structure elements (mainly α-helices) fully disappear and compared to GndHCl-induced unfolding (data not shown here) the CD signals are identical
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The addition of a small amount of Bi (1 wt
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In contrast to reports on DNA binding with monocations, the compaction potentials of monocations determined here correlate well with biological assays that describe the compaction of chromosomes
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The observation of well-resolved subpopulations in the FRET efficiency histogram indicates that the ribozyme can adopt two distinct conformations in solution
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