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In 1997, Buckham quit to answer a higher calling, establishing his own consulting firm, Alexander Strategy Group
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Drug companies justify high prices by citing a Tufts University calculation that it costs $800 million to develop a new drug and bring it to market
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4 shows quenching of GR fluorescence by iodide
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They showed that the scaling of R^sub g^ with N follows a power law of the form R^sub g^ = R^sub o^N^sup 0
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In Protein Folding Handbook, Vol
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The orientation and optimization of side chains were carried out in two steps
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9 A it is clear that it is more rapidly tumbling than the native form as indicated by the initial slope near t = 0
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Proteins were labeled with CPM by removing the reducing agents by dialysis against buffer composed of 20 mM Hepes, 0
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To test whether the heating of apoA-2:DMPC disks to 100°C followed by incubation at 24°C (where protein-DMPC binding is fastest) leads to reconstitution of intact-size disks, the sample was incubated between the consecutive DSC scans at 24°C for up to 48 h
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The former may reflect the relative ease with which a smaller protein with fewer lipid-binding helices can dissociate from the disk as compared to a larger protein with multiple, possibly cooperative lipid binding sites
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For instance, we have shown recently that, under certain conditions, the in vitro amyloidogenesis of insulin closely mimics certain aspects of the strain behavior of prion propagation (Dzwolak et al
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This will be the more likely as secondary structure forms, as we shall see by the example of a three-helix bundle below
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3 mL of horseradish peroxidase-conjugated anti-mouse IgG in the kit
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A complementary approach is to characterize the conformational distributions of freely diffusing RNA molecules using a single-molecule method referred to as diffusion spFRET (Deniz et al
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Folding of the hairpin ribozyme in its natural conformation achieves close physical proximity of the loops
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However, diminishing cellular material in 1 of the mesothelioma cases and in 10 of the cell block preparations precluded testing with TGF-[alpha]
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This study tests the hypothesis that culture within a defined chondrogenic medium containing TGF-[beta] enhances the re-expression of a chondrocytic phenotype and the subsequent production of cartilaginous extracellular matrix by human chondrocytes used in ACI
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