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All spectra were corrected for background contribution by subtracting appropriate blanks containing only buffer and GdnHCl
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N-Acyl-L-aromatic amino acid deacylase in animal tissues
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The samples were excited al 370 nm and emission scan was obtained from 420-520 nm
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It is worth mentioning here that our efforts to cleave the intact glycoprotein using α-mannosidase failed repeatedly
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A 1-cm-pathlength quart/, cell (Hellma, Muellheim/Baden, Germany) was used
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The sample was thereafter stained using 2% uranyl acetate for 20 s, and the grids were dried overnight
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3)PAL/BLxl00ED/BLxl0041
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4) are plotted as a function of residue number in Fig
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2000; Morgan, Barer, and Agnew 2003)
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