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. Although gSBA unfolded via a two-state process without the formation of any unfolding intermedialcs, the denaturation profile of rSBA was poputated with an intermediate (Fig
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. In a previous study, we probed the role of the two proximal GlcNAc residues in tethering the glycans attached to one subunit to the side chains of amino acid residues of an adjacent subunit at the noncanonical interface (18)
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. In principle all these phenomena can alter the microenvironment of the intrinsic fluorophores and would therefore affect the emission spectra
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. 15 M Tris-HCl, pH 8, followed by column chromatography (23)
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. Unique environment of Trp48 in Pseudomonas aeruginosa azurin as probed by site-directed mutagenesis and dynamic fluorescence spectroscopy
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. The Sn-Ag system limits the solid solubility of Ag in Sn, which makes it more resistant to coarsening and forms more stable as well as uniform microstructure, whereas the addition of a small amount of Cu can prevent the dissolution of Cu from the base metal
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. A different scenario must be realized in compact DNA
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. Samples were made containing 0
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. The misfolding and aggregation reaction as monitored by chemical cross-linking (Fig
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. TTR aggregates were generated from acid unfolded TTR by addition of 100 mM NaCl inducing the A-state
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. 4 C), indicating higher kinetic stability of the disks as compared to lipid-poor and vesicle-bound protein
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. The triboelectric numbers for the four toners (Colorocs, IPP, EVA 9%, and EVA 19%) were 9
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. By contrast GAG levels were reduced by 25% over the same period for pellets maintained in DMEM/10%FCS
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.Abbreviations used: CD, circular dichroism; DTNB, 5,5'-dithiobis-(2-nitrobenzoic) acid; EDTA, ethylenediaminetetracetic acid; GdnHCl, guanidine hydrochloride; GR, yeast glutathione reductase; GSH, reduced glutathione; GSSG, oxidized glutathione; HEPES, n-(2-hydroxyethyl) piperazine-n'-(2-ethanesulfonic acid); HPLC, high performance liquid chromatography; MG, molten globule
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