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. This indicates that subtle conformational changes are induced by low concentrations of GdnHCl (The existence of partially folded intermediate states in the unfolding of GR is indicated by a comparison of fluorescence spectral shift, anisotropy, iodide quenching, and CD data as a function of GdnHCl concentration
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. Blank corrections were made in all spectra
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. Nevertheless, the trends remain correct, but the ΔH^sup #^ numbers should be considered with proper care
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. The binding affinity was compared between the different probes defining an intensity 50 value, I^sub 50^, being the half height of the maximum emission intensity, corresponding to an apparent dissociation constant
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. Throughout all experiments, only ultrapure water (resistivity > 18 MΩ cm) was used
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. 95, respectively, based on the observed endpoints in the E shifts in Fig
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