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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35) professional make up artist supply .
The signal was acquired every second, and the increment of wavelength was set to 1 nm professional make up artist supply .
The other major change in a fluorescence signal upon conformational changes of the protein is related to solvent relaxation, which determines the magnitude of the stokes shift professional make up artist supply .
The similar T^sub 2^ values of the animal and vegetal methylene peaks imply that molecular motion occurs at the same timescale in both compartments and cannot account for the linewidth difference professional make up artist supply .
If the cytoplasm is dehydrated as, for example, in the desiccation of a seed, the results indicate that when the concentration of globular proteins exceeds 60% w/w this component will vitrify professional make up artist supply .
To determine the activation energy (enthalpy) E^sub a^ [congruent with] ΔH*, the Θ^sub 222^(t) data were fitted by exponentials (Fig professional make up artist supply .
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