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Although suitable data are not available to establish if there is a direct correlation between the above ANS binding capacity and the actual instability to freeze-thawing (depending on the protein, irreversible damage has been estimated by diverse criteria such as loss of enzymatic activity, degree of aggregation, and loss of secondary or tertiary structure and, further, the outcome is generally influenced by the rates of freezing and thawing), it is a common observation that multimeric enzymes tend to be less stable to freezing than their monomeric counterpart (17) personalized make up bag .
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