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. The average available volume is filled inefficiently, and cavities of all sizes are found within the interiors of denatured proteins
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. Similar results were obtained for all other two-state protein sequences shown in Table 1
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. This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein
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. 1 are all in nonhelical regions except residues 72, 73, and 75, which are pail of the less well-defined C-terminal part of helix IV
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. It is notable that although pressure techniques have been used to monitor the associations between aqueous soluble proteins, pressure has not yet been applied to monitor lateral associations of membrane-bound proteins (for recent reviews, see Ernst, 2002)
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. This erroneous model is of interest only because it has been used previously for denatured proteins in studies aimed at correlating m values and ΔC^sub P^ to changes in solventaccessible surface area (90)
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. This apparent K^sub d^, which reflects protein-protein associations on a confined membrane surface, is predicted to be ~2 orders of magnitude weaker when the proteins are free to associate in solution (see Runnels and Scarlata, 1999)
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