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. The best fitting results were obtained according to the least root mean square criterion
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. The supernatant was again subjected to 65% ammonium sulfate fractionation
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. The protein concentration used was 0
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. 5 M denaturant concentration also exibits a loss in the value of the mean residue ellipticity, suggesting that there is a loss in the secondary structure of the protein (Fig
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. The conclusion is that in the EV limit, the average shape for a protein is that of a prolate ellipsoid, i
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. Statistical mechanics of semi-Hexible chains: A mean held variational approach
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. Similarly to that observed for EIC^sup ec^, the electrostatic, as well as the van der Waals interactions, were decreased in EIC^sup sc^, losing several salt bridges and hydrogen bonds when compared with the structure of EIC^sup tt^
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. For magic angle spinning (MAS) experiments, an indirect detection pulsed field gradient nano H{X} probe (4 mm) was used in a Varian (Palo Alto, CA) UNITY INOVA 500 NMR Spectrometer
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. The plots can be interpreted in analogy to Guinier plots of polydisperse dilute solutions: In SAS experiments, linear regimes can be assigned to populations of distinct radii of gyration R^sub g^, provided Q*R^sub g^ Whereas at low temperatures, intensities decreased linearly over the whole Q-range measured for all samples, a more or less pronounced kink appeared in the Guinier plots above ~200 K (Fig
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. We gratefully thank María T
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. These results gave a volume change of 34 ± 5 ml/mol
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. Docked fractions calculated from relative areas of the peaks are plotted in Fig
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