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In a second experiment, we compared dimeric DmAChE and tetrameric HuBChE
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. The inclusion bodies thus obtained were precipitated using 65% ice-cold acetone
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. The transitions measured by CD became broader as the concentration increased, and although a small increase was observed when the concentration was raised (3 µM yielded T^sub m^ = 328
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. Stimulated by the biological importance of this subject, many in vitro investigations of DNA interaction with K+ and Na+ ions as well as other monovalent alkali cations (Li+, Rb+, and Cs+) have been performed by biophysicists (Bleam et al
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. In this work we describe the characteristics of prefibrillar and protofibrillar assemblies of TTR and present a platform for spectroscopic characterization of these species
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. TTR aggregates were generated from acid unfolded TTR by addition of 100 mM NaCl inducing the A-state
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. In summary, both the kinetic (Fig
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