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. Since the diffusion is always maintained in some low-dimensional subspace of the full folding space, τ ~ R^sup 2^/D for each subspace, so that τ^sub fold^ ≈ d^sup 2^R^sup 2^/D rather than the exponentially larger (R^sup 2^/R^sup 2^^sub N^)^sup d^
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. A peak close to zero FRET efficiency is always observed, and occurs in part due to photobleaching of the acceptor, as well as other processes that give rise to donor-only molecules (Deniz et al
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