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Finally, the three solutions of apomyoglobin in N, A, and U^sub A^ states were prepared with HCl at pH 5
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All the experiments were done in a Jobin Yvon Horiba fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm water- jacketed cell using a protein concentration of 1 µM and ANS concentration of 50 µM
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Calculation of shape parameters In Eq
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Am J Respir Crit Care Med 1997; 156:1969-1977(7) Hodge S, Hodge G, Holmes M, et al
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Science 2003; 302:1560-1563(30) Kunisaki Y, Masuko S, Noda M, et al
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Biochem Biophys Res Commun 2004: 318: 32-37(52) Huynh ML, Malcolm KC, Kotaru C, et al
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Although its physiological role is still unknown (22), it has found use as a stoichiometric bioscavenger for toxic organophosphate nerve agents (23)
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The authors thank Jacques Colletier for useful discussions on cholinesterase biochemistry as well as the IN16 instrumental team (Bernhard Frick, Thilo Seydel, and Matthias Elender) and the IN13-CRG team (Marc Bée, Francesca Natali, and Sebastien Vial) at the Institute Laue-Langevin, Grenoble, France
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EXPERIMENTAL SECTIONMaterialsBacteriophage T4 DNA (165
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11 B), amyloid fibrils of insulin, and lysozyme (P
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semifasciata and the extended time period (November-March) in which larvae were collected
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Brill, Leiden, The Netherlands
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The smallest particle size (22 µm) occurred with a 10 wt% solution of PP3825 at 130°C dissolving temperature, so we used these conditions for the subsequent tests
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Because the two loop-carrying arms are in close proximity, it is likely that the quasi-docked conformation facilitates the formation of tertiary interactions between loops A and B
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Thus, we believe that the strong expression of EGFR on cell membranes may be related to promoted cell proliferation in malignant mesothelioma
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