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Even for TAKA, which does not show any aggregation, we observed slightly smaller red shift for thermal unfolding, but due to missing aggregation still larger as compared to BLA, BAA, and BSUA eye gothic make tip up .
Most HLH proteins belong to the bHLH group, which has a basic sequence directly N-terminal to the HLH motif eye gothic make tip up .
In practice, the large difference in anisotropy decay between monomeric forms lead to a broad baseline with the contribution of the smaller monomeric forms contributing as a decay curve superimposed onto a baseline eye gothic make tip up .
This could be due to charge repulsion of positively charged ThT and protein at pH 3 eye gothic make tip up .
[1] were (1) nucleation rates for LLPS and crystallization, (2) relative position of LLPS and crystallization temperature, (3) time provided by the thermal cycle for nucleation of phases during LLPS before nucleation of crystallization occurs, (4) relative positions of spinodal and binodal regions (nucleation rate of LLPS phases), and (5) growth of phases after nucleation (especially LLPS [1, 10, 15]) eye gothic make tip up .
However, based on the observed FRET efficiency, the two loop-carrying arms appear to be relatively far apart in that species, approximately midway between the extended and docked conformers eye gothic make tip up .
Currently, we are unable to unambiguously rationalize the difference between the behavior of the immobilized 4WJ ribozyme and the 4WJ construct examined in our solution-based spFRET experiments eye gothic make tip up .
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