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. The sequence of the events was characterized by analyzing the signs of the peaks in the 2D IR correlation plots using rules proposed by Noda (29)
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. In brief, the intrinsic Trp fluorescence emission spectra were measured using a Hitachi F-2500 spectrofluoromeler (Tokyo, Japan) using 1-cm path-length cuvettes with an excitation wavelength of 295 nm
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. The solvent-accessibility of tryptophan and tyrosine residues was examined by iodide and acrylamide quenching at several pHs
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. ANS binding experiments, extended to a set of proteins including serum albumin, α-amylase, β-galactosidase, alcohol dehydrogenase from horse liver, alcohol dehydrogenase from yeast, lactic dehydrogenase, and aldolase, confirmed that a stressed condition of the native fold in the frozen state appears to be general to most proteins and pointed out that oligomers tend to be more labile than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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. Key words: Wettability, Sn-2
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. Tjandra and co-workers (3) have shown, via ^sup 15^N-relaxation at multiple fields, that in free CaM the two domains undergo a slow "wobbling" motion on a timescale of ~3 ns, apparently not evident in target-bound CaM
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