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SUPPLEMENTARY MATERIAL An online supplement to this article can be found by visiting BJ Online at http://www
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Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli
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, no significant differences in global molecular dynamics on an Ångström-nanosecond scale between native and soman-inhibited HuBChE below 334 K (61°C), but increased flexibility of the native sample with respect to its inhibited counterpart above that temperature-be reconciled with the cited studies?Results below 61° CIdentical MSDs of two samples over a temperature range as extended as 20-334 K means that the atoms of both samples are placed in common mean free-energy surfaces (47)
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In presence of internal motion, (τ^sup B^^sub m^)^sub app^ depends on the magnetic field, timescale, and degree of internal motion (22,32)
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The results of DNA compaction by metal chlorides in PEG clarify that in some cases the orders of relative binding and compaction activities can be even opposite
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Structural mapping of an aggregation nucleation site in a molten globule intermediate
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It suggests that kinetic strategies for fast folding may be encoded rather generally in nonnative as well as in native interactions
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The intermolecular interaction involves stable hydrophobic contacts with a unique protruding CD4-Phe43 structure surrounded by an intermolecular hydrogen-bond network that covers the hemisphere of the CD4 D1 domain
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Structures of HIV-1 gp120 envelope glycoproteins from laboratory-adapted and primary isolates
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Here we present a systematic study of the folding behavior of hairpin ribozymes containing different combinations of loop and helical junction elements
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For the activity assays (see below), the substrate strand sequences used are 5'-GGC GUG GUA CAU UAC CUG GUA CCC CCU CGC AGU CCU A-3' (S2WJ), 5'-GUC AAC UCG UUC GCA GUC CUA-3' (S3WJ) and 5'-GCA AGC CAC CUC GCA GUC CUA-3' (S4WJ)
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A threshold of 40 counts (sum of signals from the two channels) was then used to separate fluorescence signals from background, and FRET efficiencies were calculated for each accepted event using the equation E = (1 + [gamma]I^sub d^/I^sub a^)^sup -1^
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. The results of DNA compaction by metal chlorides in PEG clarify that in some cases the orders of relative binding and compaction activities can be even opposite
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.Received 13 January 2003; Accepted after revision 3 February 2004
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