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. In this research, the sequential events in ACYI thermal denaturation were investigated by a combination of spectroscopic methods and related resolution-enhancing techniques
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. In general, the thermal denaturation of proteins can be demonstrated by a two-state thermodynamic mechanism
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. It can be concluded, then, that EI^sup sc^ at low pH unfolded via a moltenglobule-like species, as it has been described in other large multimeric proteins (49)
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. The distortion of the native structure inferred from ANS binding was found to draw a parallel with the extent of irreversible denaturation by freeze-thawing, suggesting that these altered conformations play a direct role on freeze damage
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. ACYI is, to some extent, a thermostable enzyme with an optimal temperature for activity at ~65°C
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. We conclude that the modifications of HuBChE molecular dynamics on an Angstrom-nanosecond scale upon covalent binding of the small inhibitor soman are, if present, either local (e
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.Solder and FluxesIn this experiment, Sn-2
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. Hammarström, unpublished) compared to binding at pH 7
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. Toward an outline of the topography of a realistic protein folding funnel
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