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. Due to a tight sealing of the quartz cuvettes temperatures of protein solutions up to ~120°C were applicable without evaporation and boiling
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. It is obvious that this also can have different effects on the microenvironment of tryptophan residues
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. The three-dimensional structure of BChE is very similar (21)
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. Thus, CaM can, in a Ca^sup 2+^-dependent manner, directly interact with some members of the bHLH family
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. Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M)
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. Here τ^sub 1^, τ^sub 2^ are the exponential relaxation times, A^sub 1^, A^sub 2^ are the amplitudes, and A^sub 0^ is the value of Θ^sub 222^ at t [arrow right] ∞
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. The T-jump data to 95°C are well approximated by a single exponential, yet two exponents are required to fit the data at 75-90°C, suggesting two kinetic phases
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. Financial support from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie and the COST D30 action "High Pressure Tuning of Biochemical Processes: Protein Dynamics and Aggregation" is gratefully acknowledged
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