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The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1) clothing drag make queen up .
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At this pH the protein has a net negative charge, the dimer carrying a charge of -17e (36), where e is the protonic charge; and so the apparent specific volume would be expected to have a contribution from net repulsive electric double layer interactions in addition to the excluded volume of the hydrated protein itself clothing drag make queen up .
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