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. The results are shown in Fig
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. Here, we report that binding of the fluorescence probe 1-anilino-8-naphthalene sulfonate (ANS) to proteins in ice can provide a general monitor of ice-induced alterations of their tertiary structure
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. We also determined the pressure range in which the proteins remain membrane bound: PLCβ binding was stable throughout the 1-2000 bars range, Gβγ binding was stable only at high membrane concentrations, whereas Gagr;^sub s^(GDP) dissociated from membranes above 1 kbar
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. We also tested the ability of Gα^sub s^ to remain membrane-bound under pressure
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. and light scattering in this (Fig
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. These enzymes deplete the polar HDL surface of the proteins or phospholipids and/or increase the apolar lipid core, thereby creating a mismatch between the particle surface and its core (clay et al
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. Since the diffusion is always maintained in some low-dimensional subspace of the full folding space, τ ~ R^sup 2^/D for each subspace, so that τ^sub fold^ ≈ d^sup 2^R^sup 2^/D rather than the exponentially larger (R^sup 2^/R^sup 2^^sub N^)^sup d^
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. In one series of DSC experiments, the heat capacity C^sub p^(T) was recorded from the same sample during repetitive heating from 5 to 100°C followed by incubation at 24°C for 0-48 h (Fig
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