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. Hence, not many orientations of the glycan chain upon attachment to the protein are possible and we assume that upon energy minimization, the structure obtained mimics the real situation
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. Trial moves that set backbone torsions are pivot moves because these lead to large-scale conformational changes
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. This paradigm forms the basis for the use of tri- and pentapeptides, coil libraries (125,128), and fragments excised from structural databases for modeling properties such as solvent-accessible surface areas (129) in the highly denatured state
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. The qualitative structural implication is, therefore, that the second CaM monomer in the dimeric complex is placed along this axis in such a way that the tips of the N- and C-terminal domains touch each other
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. With increasing mass fraction, the expected increase in η^sub r,0^ with φ is less than expected
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. The configurational entropy analysis in terms of combined trajectories presented here shall potentially provide accurate estimations of thermodynamic properties of biomolecules given sufficient sampling of conformational space
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. DISCUSSIONSufficient conformational sampling is a prerequisite for a reliable estimation of the conformational entropy
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. When different τ^sub is^ values were tested, the lowest χ^sup 2^ values and hence the best overall fit was found for τ^sub is^ equal to 2
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