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The overexpressed dimeric enzyme has four glycosylation sites per monomer, with carbohydrates accounting for ~10% of the dry weight barbers hill isd .
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4, A and B), and the all-atom values are still in the buildup phase throughout the 8-ns trajectories (Fig barbers hill isd .
Although less confined than CD4-F43, it is in close contact (In addition to the residues that are directly involved in intermolecular contacts or are in close proximity to the intermolecular interface, there are a few residues in gp120 that show large intraresidue entropy changes upon complexation (see Fig barbers hill isd .
For the activity assays (see below), the substrate strand sequences used are 5'-GGC GUG GUA CAU UAC CUG GUA CCC CCU CGC AGU CCU A-3' (S2WJ), 5'-GUC AAC UCG UUC GCA GUC CUA-3' (S3WJ) and 5'-GCA AGC CAC CUC GCA GUC CUA-3' (S4WJ) barbers hill isd .
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