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Spectral changes were found to be reversible upon removal of the denaturant by dialysis (data not shown)
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Although the glycosylated form undergoes a simple two-state unfolding, the nonglycosylated species unfolds via a compact monomeric intermediate that is not a molten globule
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RESULTS Biophysical characterization of the rSBA The biophysical characteristics of the rSBA are very much similar to that of the native SBA obtained from the seeds of Glycine max
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This suggests that proteins in the EV limit have an average ellipsoidal shape
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The mean residue ellipticity, [Θ], was obtained from the raw ellipticity data, Θ, as described (23), and all spectra were corrected with the proper baseline
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The slit widths were 5 nm for the excitation and emission wavelengths
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Nature 2005; 434:93-99(33) deBakker CD, Haney LB, Kinchen JM, et al
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Indeed, several lines of evidence indicate that both pressure-induced and thermal denaturation of HuBChE are multistep processes that are initiated by penetration of water into the active-site gorge, leading to protein swelling and, eventually, unfolding (18,53,54)
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Negative wetting force, i
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1, the osmotic pressure data indicate that the dominant interaction between the protein particles is repulsive
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* Pat Hand--A hand with a total of 17 to 21
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President Bush has directly occupied two countries in the region and threatened a third
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