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However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the α-amylases with red shifts upon thermal unfolding (i
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At small strains, γG' over the frequency range examined of 0
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Therefore, with regard to DNA monomolecular compaction, it is important to consider morphological effects that result from the dramatic change in the molecular density of DNA
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A total of 25 µl 6 × SDS sample buffer (final SDS concentration of 2
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The C^sub p^(T) data were recorded from degassed protein and/or buffer solutions upon repetitive heating from 5 to 100°C at a rate 90 K/h, followed by cooling and incubation at 24°C
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The microscope was coupled to an AS-12 E-scanner (13-µm) and an Extender Electronics Module EX-II (Digital Instruments, Santa Barbara, CA), which allows acquisition of phase images
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Structural analysis of the rate-limiting transition states in the folding of Im7 and Im9: similarities and differences in the folding of homologous proteins
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Gut length was moderate (PAL/BL 0
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semifasciata resembled the larvae of other pinguipedids in their gut size, meristics, and general pattern of pigmentation
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The melt flow rates for the EVA 9% were greater than 0
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If instead, subpopulations interconvert rapidly on the burst-duration timescale, a single averaged peak will be observed, with increased broadening as the interconversion rate approaches this timescale
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Lastly, kinetic information about equilibrium interconversion of subpopulations (on microsecond to millisecond timescales) is uniquely available from spFRET data
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. Between 2 and 5 M GdnHCl, a significant drop in anisotropy was observed, indicating faster rotational mobility of tryptophan residues upon unfolding of GR
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.Folding of single-loop ribozyme constructsThe results described in the preceding section suggest that Mg^sup 2+^-induced conformational changes within the helical junction region may control the approach of loops A and B during ribozyme folding
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. This result confirms that the sizable increases in E with Mg^sup 2+^ that we observe for the 2WJ (WT and SL) and SL 4WJ ribozymes are due to decreases in the interarm distances
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.Evidence for a unique folding intermediate in the 4WJ ribozymeOur results indicate that the SL 4WJ ribozyme can fold into a compact conformation in the presence of high Mg^sup 2+^ concentrations, with arms A and B juxtaposed in a similar fashion to the docked native 4WJ ribozyme
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