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. DISCUSSIONMolten globule (MG) folding intermediates have been characterized for a number of proteins (for reviews, see Kuwajima, 1989; Ptitsyn, 1992, 1995)
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. Thermodynamic study of the apomyoglobin structure
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. Both a greater affinity and a larger number of binding sites attest to a significantly greater perturbation of tertiary structure in the azurin mutants than in the WT
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. , under conditions used for generating the FRET histograms in this article)
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. Pyruvate (1 mM), dexamethasone (10^sup -7^ M) and 10 ng/ml TGF-[beta]1 (recombinant human, R&D Systems, Abingdon, UK) were also added
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