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. Yeast GR presents a quite blue-shifted fluorescence emission spectrum (maximal emission wavelength at 317 nm for the native protein; see Fig
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. When considering all protein atoms, the number of atoms in gp120 becomes too large to obtain sufficient sampling in the timescale of our simulation (10 ns); the buildup curves have not yet converged (Fig
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. Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature
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