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Yeast GR presents a quite blue-shifted fluorescence emission spectrum (maximal emission wavelength at 317 nm for the native protein; see Fig applying make up .
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Quantitative estimation of protein binding site polarity applying make up .
In the nucleus, the signal appeared to be dominated by water, whereas other contributions were negligible applying make up .
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It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs applying make up .
High pressure dissociated PLCβ-Gβγ with a ΔV = 34 ± 5 ml/mol applying make up .
These naturally belong to the levels of coarse-graining at which we treated the helix bundles above applying make up .
When considering all protein atoms, the number of atoms in gp120 becomes too large to obtain sufficient sampling in the timescale of our simulation (10 ns); the buildup curves have not yet converged (Fig applying make up .
About 50 mL of antibody solution was obtained after two weeks of cultivation applying make up .
Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature applying make up .
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