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A total of 27 hydrogen-bonding interactions and 53 hydrophobic interactions were found to stabilize the structure, which suggests that hydrophobic interactions between the protein and the glycans are responsible for the exceptionally high stability of the glycoprotein application make tip up .
all histidine residues were solvent-exposed: and application make tip up .
Although for BSA a high binding capacity may be expected from its intrinsic tendency to bind nonpolar and aromatic moieties also in the liquid state, the above ANS binding data do suggest that beyond specific trends of individual polypeptides, multi-subunit proteins are more susceptible than monomers to destabilization and partial unfolding in the frozen state application make tip up .
In both cases, the rate of increase of the MSDs decreases drastically at the highest measured temperatures (T > 270 K), as observed previously (44) application make tip up .
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We have compared the characteristics of the CaM:SEF2-1 mp interaction with those of other CaM:protein complexes and other protein:protein complexes application make tip up .
1), the calculated R^sub h^ of both the protein in solution and its aggregate increased in size and was correlated to the phase separation/precipitation of BLG from aqueous solution at these concentrations and ionic strength (34) application make tip up .
ThT does not show binding to the U-state and only shows a minor burst phase, indicating that this is a clearcut aggregation probe application make tip up .
2 A), which result from the negative staining application make tip up .
The neighboring residue Q40, which lies at the center of the complex interface, shows the largest reduction among all residues (top row in Fig application make tip up .
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For each of these identified points, the constituent 50-µs binned data was then used to calculate the "burst duration" during this time bin, by determining the total time spent above a threshold of 4 counts application make tip up .
Beyond kinetic traps in RNA folding application make tip up .
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