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. Blindness in Astyanax seems to be caused instead by mutations in a small number of master switches that control other genes and proteins necessary to the development of the eye
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. Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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. This paradigm forms the basis for the use of tri- and pentapeptides, coil libraries (125,128), and fragments excised from structural databases for modeling properties such as solvent-accessible surface areas (129) in the highly denatured state
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. Further support for this hypothesis comes from a large reduction in the apparent T^sub m^ and faster thermal unfolding observed upon disulfide reduction in apoA-2:DMPC disks (data not shown), which is consistent with the report that apoA-2 on the disks is less stable in monomeric than in dimeric form (Lund-Katz et al
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