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Sample solutionsSolutions for microscopic observations were prepared by mixing PEG 3000 or PEG 10000 in twice-distilled water until dissolution, adding T4 DNA with further mixing and incubation for 30 min, adding DAPI with further mixing and incubation for 30 min, and finally adding alkali chloride solution and letting the sample solution sit for 1-2 h before observations
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Circular dichroism (CD) and light-scattering melting curves show hysteresis and scan rate dependence, indicating thermodynamically irreversible transition with high activation energy E^sub a^
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ACKNOWLEDGEMENTSThe authors would like to thank Bradley Wood, Chrystal Pierce, Anette Herrera and Shoreh Biazar-Sadri for their help with data collection
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