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Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^
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Neutrophil elastase inhibits PS-mediated ingestion, presumably by cleaving an unidentified PSR and/or the antigenic target of Mab217
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Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte
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In an attempt to correlate affinities and n values to specific aspects of the macromolecule, we observe that all mutants are less stable than the WT by a comparable amount, the denaturation-free energy change, ΔG° at 20°C, decreasing from 6-7 to ~3 kcal/mole (20,27)
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0 do not support this explanation (Fig
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11 B), amyloid fibrils of insulin, and lysozyme (P
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The use of high pressure also allowed us to determine the volume change associated with the interaction between Gβγ and PLCβ
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Concomitantly, the third hypervariable loop (V3) undergoes a rearrangement to a somewhat different conformation leading to accessibility of the epitope for co-receptor binding
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This indicates that the intermolecular interactions between gp120 and CD4, although decreasing the intraresidue entropy of residues in direct contact, can induce intraresidue entropy changes at the interdomain interface within the gp120 monomer and at the putative trimeric gp120 interface
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Figure 3 shows the optimized steps for producing toner by TIPS and subsequent solvent exchange
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Taken together, the smooth E shifts with changing Mg^sup 2+^ concentration combined with the observed broadening/ tailing at high Mg^sup 2+^ concentration are consistent with the presence of a dynamic equilibrium between the quasi-docked state and an extended undocked state, with a relatively small energy barrier for interconversion (Fig
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Luck of the dice: Gambling attitudes of a sample of community college students
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