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. Chemical modification of two tryptophan residues abolishes the catalytic activity of aminoacylase
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. We observe that the unfolding of gSBA is a two-state process
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. 2 M lactose in 5 mM HEPES buffer, pH 7
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. The readings were obtained at pH 7
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. Similar results were obtained for all other two-state protein sequences shown in Table 1
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. Here, we compare the ANS binding capacity of the four azurin species to inquire how these modified features modulate the ice-induced perturbation of tertiary structure
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. Backbone dynamics and energetics of a calmodulin domain mutant exchanging between closed and open conformations
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. , 60%) concentrations of Gβγ on lipid membrane and at supersaturating concentrations of Gβγ are shown in Fig
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. Interestingly, the activation energy E^sub a^ = 60 ± 8 kcal/mol of the slow heat-unfolding phase in apoA-2:DMPC disks (Fig
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. In the case of NC flux shown in Fig
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. This mutation eliminates a tertiary hydrogen bonding interaction between G^sub 11^ (loop A) and A^sub 23^ (loop B) and is expected to destabilize the docked conformer (Rupert and Ferrc-D'Amare, 2001; Klostermeier and Millar, 2002)
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