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. The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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. Our ability to simulate mimics of denatured-state ensembles will allow us to ask precise questions about the role of the denatured state in the types of applications outlined above
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. Direct characterization of the folded, unfolded, and urea-denatured states of the C-terminal domain of the ribosomal protein L9
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. Our approach suggests that thermodynamical analysis might reveal subtle conformational changes
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. The Principles of Nuclear Magnetism
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. Such molecular systems were first developed for the characterization of free-volume concepts in polymers because of their unique sensitivity to torsional rigidity of the surrounding medium (Loutfy, 1986)
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. The reason for these differences is not known but may be related to technique, including antigen retrieval, types of antibodies used, or both
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.Fluorescence anisotropy measurements were performed in the same instrument with polarizers in the excitation and emission ports
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